Scaffold gap completion

http://edamontology.org/operation_3217

Fill the gaps in a sequence assembly (scaffold) by merging in additional sequences.

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operations, edam

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1.1

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Different techniques are used to generate gap sequences to connect contigs, depending on the size of the gap. For small (5-20kb) gaps, PCR amplification and sequencing is used. For large (>20kb) gaps, fragments are cloned (e.g. in BAC (Bacterial artificial chromosomes) vectors) and then sequenced.

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Scaffolding